Intermolecular interactions in soft matter; chemical force microscopy; bio-nano-photonics; analytical microdevices; controlled synthesis and assembly of materials at meso-scale .
In our research, we set the goal of understanding and controlling interactions in chemical systems at small scale (microns to nanometers). The research is interdisciplinary, where approaches of chemistry, physics and engineering converge. We pursue three major directions in this area:
1. Bio-nano-photonics. We interested in developing new experimental methodology to characterize mechanisms and dynamics of intra- and intermolecular binding that involves biomolecules. In particular, force spectroscopy can manipulate single molecules and perform mechanical experiments with single macromolecules, thus, one can move beyond ensemble averages to resolve and analyze transient subpopulations, study details of energy landscapes, and observe mechanics of molecular machinery of cells. To gain independent evaluation of geometrical, structural and molecular transformations within the probe-substrate junction, we combine force spectroscopy with optical near field techniques to achieve simultaneous spectroscopic characterization of binding and folding events. Nanostructured substrates (sub-wavelength apertures, lenses or waveguides) will act as field concentrators to achieve light confinement to the nanometer-sized region of interest.
2. New tools for research in biophysics and bioengineering. Modern scanning probe microscopy techniques are very powerful tools for characterization of molecular properties and function at the nanometer scale; however, complexity and high cost prevent their widespread use in chemical and biological laboratories. We are exploring new, simple approaches to the analytical force spectroscopy platform for massively-parallel low cost binding assays.
3. Meso-scale assembly of functional nanomaterials. One current challenge for science of nanometer scale materials is to provide reproducible and practical routes to assembling these materials into functional devices - a problem which involves making interconnects between macro scale (mm to microns) and nanometer scale. We are developing tools and approaches to three-dimensional self-assembly of semiconductor nanowires during growth, and research their potential applications in photonics and sensors.
Physical chemistry, surface and colloidal chemistry, biophysical chemistry, analytical chemistry, fundamentals of science and technology at the nanometer scale
Cheng, P.; Barrett, M.J.; Oliver, P.M.; Cetin, D.; Vezenov, D. “Dielectrophoretic Tweezers as a Platform for Molecular Force Spectroscopy in a Highly Parallel Format” Lab Chip, 2011, 11, 4248 - 4259.
Wagner, K.; P. Cheng; Vezenov, D.V. “Non-contact Method for Calibration of Lateral Forces in Scanning Force Microscopy” Langmuir, 2011, 4635-4644.
Gan, Q.; Gao, Y.; Wagner, K.; Vezenov, D.V.; Ding, Y.; Bartoli, F. "Experimental verification of the "rainbow" trapping effect in adiabatic plasmonic gratings" Proc. Natl. Acad. Sci., 2011, 5169-5173.
Yin, P.; Wu,P.; Xiao,Z.; Li, D.; Bitterlich, E.; Zhang, J.; Cheng, P.; Vezenov, D.V.; Liu,T.; Wei, Y. “A double-tailed, fluorescently active surfactant with hexavanadate as giant polar head group”, Angewandte Chemie, 2011, 2521-2525.
Oliver, P.M.; Park, J.S.; Vezenov, D.V. “Single Molecule Force Spectroscopy using Magnetic Tweezers with Evanescent Illumination: Quantitative High-Resolution Sensing of DNA Hybridization”, Nanoscale, 2011, 3, 581–591.
Bijamov, A.; Shubitidze, F.; Oliver, P.M.; Vezenov, D.V. “Quantitative Modeling of Forces in Electromagnetic Tweezers”, J. Appl. Phys., 2010, 108, 104701-1--104701-9.
Lim, H.; Marzillier, J.; Vezenov, D.V. “Heterobifunctional Modification of DNA for Conjugation to Solid Surfaces”, Anal. Bioanal. Chem., 2010, 397, 1861–1872.
Bijamov, A.; Shubitidze, F.; Oliver, P.M.; Vezenov, D.V. “Optical Response of Magnetic Fluorescent Microspheres Used for Force Spectroscopy in the Evanescent Field”, Langmuir, 2010, 26, 12003–12011.
Fuller, C. W.; Middendorf, L.R.; Benner, S.A.; Church, G.M.; Harris, T.; Huang, X.; Jovanovich, S.B.; Nelson, J.R.; Schloss, J.A.; Schwartz, D.C.; Vezenov, D.V. “Challenges of Sequencing by Synthesis”, Nature Biotech., 2009, 27, 1013-1023. Perspective article.
Manohar, S.; Mantz, A.; Bancroft, K.; Hui, C.-Y.; Jagota, A.; Vezenov, D.V. “Peeling Single Stranded DNA from Graphite Surface to Determine Oligonucleotide Binding Energy by Force Spectroscopy” Nano Lett., 2008, 8, 4365-4372.
Vezenov, D.V.; Noy, A.; Lieber, C.M “Chemical Force Microscopy: Force Spectroscopy and Imaging of Complex Interactions in Molecular Assemblies.” in Handbook of Molecular Force Spectroscopy, A. Noy (Ed.), Springer, 2008, pp. 123-143.
Noy, A.; Vezenov, D.V.; Lieber, C.M “Chemical Force Microscopy: Nanoscale Probing of Fundamental Chemical Interactions.” in Handbook of Molecular Force Spectroscopy, A. Noy (Ed.), Springer, 2008, pp. 97-122.
Vezenov, D.V.; Noy, A.; Ashby, P. "Chemical force microscopy: probing chemical origin of interfacial forces and adhesion." (Invited) special issue of J. Adhesion Sci. Technology , 2005.
Vezenov, D.V.; Mayers, B.T.; Conroy, R.; Snee, P.; Chan, Y.; Bawendi, M. G.; Whitesides, G. M. "A low-threshold, high-efficiency microfluidic waveguide laser". ASAP article, J. Am. Chem. Soc. , 2005.
Xu, Q.; Mayers, B.T.; Lahav, M.; Vezenov, D.V.; Whitesides, G. M. "Approaching zero: using fractured crystals in metrology for replica molding." J. Am. Chem. Soc. , 2005, 127 , 854.
Vezenov, D.V.; Mayers, B.T.; Wolfe, D.B.; Whitesides, G. M. "Integrated fluorescent light source for optofluidic applications." Appl. Phys. Lett. , 2005, 86 , 041104.
Vezenov, D.V.; Noy, A.; Lieber, C.M. "The effect of liquid induced adhesion changes on the interfacial shear strength between self-assembled monolayers." J. Adhesion Sci. Technology , 2003, 17 , 1385.
Vezenov, D.V.; Zhuk, A.V.; Whitesides, G.M.; Lieber, C.M. "Chemical force spectroscopy in heterogeneous systems: intermolecular interactions involving epoxy polymer, mixed monolayers and polar solvents." J. Am. Chem. Soc. , 2002, 124 , 10578.